All posts by Postępy Mikrobiologii

Promieniowce – występowanie i wytwarzanie związków biologicznie czynnych

Actinomycetes – occurrence and production of biologically active compounds
J. Solecka, J. Ziemska, A. Rajnis z, A. Laskowska, A. Guśpiel

1. Wstęp. 2. Bioaktywne metabolity promieniowców. 3. Miejsca występowania promieniowców. 4. Promieniowce bytujące w glebie. 5. Promieniowce w środowisku morskim. 6. Inne źródła. 7. Podsumowanie

Abstract: Actinomycetes are prolific producers of many bioactive metabolites, including antibacterial, antifungal, antiviral or anticancer substances. They belong to Gram-positive bacteria and are isolated from different environments. Among actinomycetes, the Streptomyces genus plays a major role in productivity of metabolites with biological activity and is most widespread all over the world. From the beginning of golden era of antibiotics, actinomycetes metabolites were mainly isolated from the soil. As the obtaining the previously discovered metabolites from terrestrial habitats increases, there are attempts to look for new sources, e.g. seas, oceans, etc. Marine isolates are different from soil actinomycetes in their chemical structures, mode of action or biological activity. Marine sponges are especially rich in actinomycete strains. However, actinomycetes are also isolated from fallen leaves, ants’ nests, deserts, Antarctica sediments and snow cores, caves or spider materials.

1. Introduction. 2. Bioactive metabolites from actinomycetes. 3. Actinomycetes in different environments. 4. Soil actinomycetes. 5. Actinomycetes in marine environment. 6. Other sources. 7. Summary

Rola czynników zakaźnych w chorobach neurodegeneracyjnych

Role of infections factors in neurodegenerative diseases
M. Wiciński, P. Sopońska, B. Brzoszczyk, B. Malinowski, E. Grześk, A. Michalska

1. Wprowadzenie. 2. Choroba Alzheimera. 2.1. Chlamydophila pneumoniae. 2.2. Wirus opryszczki pospolitej typu 1. 2.3. Krętki. 2.4. Helicobacter pylori. 3. Choroba Parkinsona. 3.1. Zespół nabytego niedoboru odporności (AIDS). 3.2. Toxoplasma gondii. 4. Stwardnienie rozsiane. 4.1. Wirus Epsteina-Barr. 4.2. Wirus Torque teno. 4.3. Ludzki Herpeswirus typu 6. 4.4. Chlamydophila pneumoniae. 5. Podsumowanie

Abstract: Pathogens are possible risk factors for chronic degenerative diseases of the nervous system. This paper summarizes the evidence of infectious agents’ relationship with neurodegenerative diseases and current knowledge about the role of pathogens in the modulation of immunological mechanism in the most common degenerative diseases: Alzheimer disease, Parkinson disease and multiple sclerosis. Infections with Chlamydia pneumoniae, Herpes simplex virus type 1, spirochaete and Helicobacter pylori may constitute risk factors for the Alzheimer disease. Human immunodeficiency virus and Toxoplasma gondii in the brain can cause parkinsonism. Epstein-Barr virus and co-infection with Torque teno virus as well as Human herpes virus type 6 and  Chlamydia pneumoniae may play a role in the pathogenesis of multiple sclerosis.

1. Introduction. 2. Alzheimer’s disease. 2.1. Chlamydophila pneumoniae. 2.2. Herpes simplex virus 1. 2.3. Spirochetes. 2.4. Helicobacter pylori. 3. Parkinson’s disease. 3.1. Acquired  immunodeficiency syndrome (AIDS). 3.2. Toxoplasma gondii. 4. Multiple sclerosis. 4.1. Epstein- -Barr virus. 4.2. Torque teno virus. 4.3. Human Herpesvirus 6. 4.4. Chlamydophila pneumoniae. 5. Summary

Mimiwirus APMV, mamawirus oraz jego wirofag – budowa i charakterystyka

APMV, Mimivirus mamavirus and its virophage – structure and characteristic
B. Tokarz - Deptuła, J. Śliwa - Dominiak, M. Kubiś, W. Deptuła

1. Wprowadzenie. 2. Charakterystyka wirusa APMV. 3. Mimiwirus APMV, a super-rodzina NCLDV (nucleocytoplasmic large DNA viruses). 4. Wirofag mamawirusa. 5. Podsumowanie

Abstract: This paper describes APMV Mimivirus, which belongs to the NCLDV super-family. The representatives of this super-family shed light a lot of biological secrets. The role of this virus is not known and this fact forms the basis for the discussion about common origin of viruses and eukaryote. In this paper a new, not yet registered virophage is presented.

1. Introduction. 2. Characteristic of APMV virus. 3. Mimivirus and super-family of NCLDV (Nucleocytoplasmic Large DNA Viruses). 4. Mamavirus virophage. 5. Summary

Koproskopowe metody ilościowe w weterynaryjnej diagnostyce parazytologicznej zastosowanie i problemy w szacowaniu ich skuteczności

Coproscopical quantitative methods in the parasitological diagnosis – the use and problems with estimation of their efficiency
M. Kochanowski, J. Karamon, J. Dąbrowska, T. Cencek

1. Wprowadzenie. 2. Przykładowe ilościowe metody parazytologiczne. 3. Przygotowanie preparatu do bad0ania. 4. Izolacja form rozwojowych pasożytów. 5. Sposób obliczania form pasożytniczych pod mikroskopem świetlnym. 6. Właściwości badanego materiału. 7. Sposoby oceny parametrów charakteryzujących metody parazytologiczne. 8. Podsumowanie

Abstract: The article is review about various aspects of coproscopical diagnostic methods. It describes selected methods and factors having significant influence on their efficiency, including preparation of the specimen, methods of isolation of developmental parasitic forms and calculating them under a microscope, the influence of feaces and parasitic forms properties and methods of calculating the multiplication factor to estimation the number of parasitic forms in 1 g of sample. In particular, it discusses problems associated with estimation the efficiency of these methods and calculation of the real content of the parasitic forms in the sample.

1. Introduction. 2. Examples of quantitative parasitological methods. 3. Preparation of specimen to investigation. 4. Isolation of developmental parasitic forms. 5. Methods for calculation of parasitic forms using the light microscop. 6. Properties of the investigated material. 7. The ways of estimation of the parameters characterizing the parasitological methods. 8. Summary

Mikrobiologiczny rozkład kwasu cynamonowego i jego hydroksypochodnych

Microbiological degradation of cinnamic acid and its hydroxyl-derivatives
D. Wojcieszyńska, K. Hupert - Kocurek, U. Guzik

1. Wprowadzenie. 2. Przemiany kwasu cynamonowego i jego hydroksypochodnych w warunkach beztlenowych. 3. Rozkład kwasu cynamonowego i jego hydroksypochodnych w warunkach tlenowych. 4. Biotransformacja kwasu cynamonowego i jego hydroksypochodnych. 5. Podsumowanie

Abstract: Microbiological degradation of cinnamic acid and its hydroxyl-derivatives occurs via aerobic or anaerobic pathway. The first step in the biodegradation of these compounds, both aerobic and anaerobic, is β-oxidation. The key intermediate in this process is benzoyl-CoA. In anaerobic environment this intermediate can be transformed to acetyl-CoA, incorporated into the central metabolism. Under aerobic condition benzoyl-CoA is transformed to protocatechuate acid, gentisic acid or catechol, compounds which are cleaved by a specific dioxygenase. Many microorganisms can transform phenolic acid to an amino acid or other compounds such as which 4-hydroxybenzoic acid, amide, acetophenone, which can be used in industry.

1. Introduction. 2. Anaerobic degradation of cinnamic acid and its hydroxyl-derivatives. 3. Degradation of cinnamic acid and its hydroxyl-derivatives under aerobic conditions. 4. Bioconversion of cinnamic acid and its hydroxyl-derivatives. 5. Summary